Rapid method for the identification of essential genes in Staphylococcus aureus.
نویسندگان
چکیده
A strategy based on a vector host-dependent for autonomous replication, pSA3182, was utilized both for the rapid screening for Staphylococcus aureus genes essential for cell viability and for the introduction of specific polarity-neutral deletions in nonessential genes. The results obtained support the use of pSA3182 for both purposes.
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ورودعنوان ژورنال:
- Plasmid
دوره 42 2 شماره
صفحات -
تاریخ انتشار 1999